Ames Plate Incorporation Assay Kits

• 31-100.2 – Salmonella Mutagenicity Complete Test Kit.

  Includes the materials needed to perform the Ames Mutagenicity Plate Incorporation assay.
  • STDisc™ TA1535, TA1537, TA98 & TA100 (71-097aL through 71-102L)
  • Lyophilized S9 (11-01L.2 x 2)
  • NADPH REGENSYS™ (60-200.4 & 60-201.4L; 40 ml sizes)
  • ControlChem™ ICR191 (60-101), Daunomycin (60-102), Sodium Azide (60-103) & 2-Aminoanthracene (60-107)
  • Nutrient Broth (26-505.1; 100 ml)
  • Histidine/Biotin Top Agar (26-503.1; 4 x 100 ml)
  • ST Quad PC™ plates (21-200)
  • Minimal Glucose Agar plates (21-400.2 x 8; 160 dishes)
  • Nutrient Agar plates (21-100; 20 dishes)
  • Application and Instruction Manual

• 31-101 – E. coli Mutagenicity Test Kit.

  Similar to the Salmonella Mutagenicity Complete Test Kit but with E. coli WP2 strains (trp and trp, uvrA) More tools here >>
  • ECDisc™ WP2 trp (72-187L) and WP2 trp uvrA (72-188L)
  • Lyphophilized S9 (11-01L.2)
  • NADPH REGENSYS™ (60-200.15 & 60-201.15L; 15 ml sizes)
  • ControlChem™ Methyl methanesulfonate (60-108) & 2-Aminoanthracene (60-157.2)
  • Nutrient Broth (26-505.1; 100 ml)
  • Tryptophan Top Agar (26-502.1; 2 x 100 ml)
  • EC Tri PC™ plates (21-199)
  • Nutrient Agar plates (21-100; 20 plates)
  • Minimal Glucose Agar plates (21-400.2 x 4)
  • Application and Instruction Manual

Microtiter Fluctuation Assay Kits

• 31-300 - Moltox FT™ TA98 & TA100 Mutagenicity Assay Kit.

  Consists of materials to perform a microtiter fluctuation test using Salmonella typhimurium strains TA98 and TA100. Includes sufficient material to perform triplicate analyses of 6 concentrations of a test material, positive and negative controls, with and without S9. More tools here >>
  
  
  • STDisc™s TA98 & TA100 (71-098L.2 & 71-100L.2; 2 discs per strain/ vial)
  • Moltox FT™ Growth Medium (26-712.05; 50 ml)
  • Moltox FT™ Exposure Medium (26-710.05; 50 ml)
  • Moltox FT™ Reversion Indicator Medium (26-711.15 x 2)
  • MUTAZYME™ Moltox FT™ 30% S9 Mix (11-401.3L; 3.25ml)
  • 2-Aminoanthracene (60-157; 100 µg/vial, 1 vial)
  • 4-Nitroquinoline-N-oxide (60-159; 50 µg/vial, 1 vial)
  • 2-Nitrofluorene (60-161; 50 µg/vial, 1 vial)
  • User Instructions

• 31-301 - Moltox FT™ “471” Mutagenicity Assay Kit.

  Contains the products required to perform a microtiter fluctuation test using Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, & an Escherichia coli WP2 strain. Includes sufficient material to perform triplicate analyses of 6 concentrations of a test material, positive and negative controls, with and without S9. Assay consistent with OECD 471. More tools here >>
  
  
  • STDisc™ TA98, TA100, TA1535, TA1537, & ECDisc™ EC WP2 (trp, uvrA, pKM101; 71-098.2L, 71-100.2L, 71-1535.2L, 71-1537.2L, 72-188.2L; 2 discs per strain/vial)
  • Moltox FT™ Growth Medium (26-712.1; 100 ml)
  • Moltox FT™ Exposure Medium (26-710.1; 100 ml)
  • Moltox FT™ Reversion Indicator Medium (26-711.5; 2 x 500 ml)
  • MUTAZYME™Moltox FT™ 30% S9 Mix (11-401.6L; 6.5 ml)
  • 2-Aminoanthracene (60-157; 100 µg/vial, 1 vial)
  • 2-Aminoanthracene (60-157.2; 2 mg/vial, 1 vial)
  • 9-Aminoacridine hydrochloride (60-158; 500 ug/vial, 1 vial)
  • 4-Nitroquinoline-N-oxide (60-159; 50 µg/vial, 1 vial)
  • N4-aminocytidine (60-160; 2.5 mg/vial, 1 vial)
  • 2-Nitrofluorene (60-161; 50 µg/vial, 1 vial)
  • User Instructions

• 31-302 - Moltox FT™ E.coli Mutagenicity Assay Kit.

  Consists of materials to perform a microtiter fluctuation test using Escherichia coli WP2 (trp, pKM101) and WP2 (trp, uvrA). Includes sufficient material to perform triplicate analyses of 6 concentrations of a test material, positive and negative controls, with and without S9 More tools here >>.
  
  
  • ECDisc™ EC WP2 (trp, pKM101) & EC WP2 (trp, uvrA); 72-002.2L & 72-188.2L (2 discs per strain vial)
  • Moltox FT™ Growth Medium (26-712.05; 50 ml)
  • Moltox FT™ Exposure Medium (26-710.05; 50 ml)
  • Moltox FT™ Reversion Indicator Medium (26-711.15 x 2)
  • MUTAZYME™ Moltox FT™ 30% S9 Mix (11-401.3L; 3.25 ml)
  • 2-Aminoanthracene (60-157.2; 2 mg/vial, 1 vial)
  • 4-Nitroquinoline-N-oxide (60-159; 50 µg/vial, 1 vial)
  • User Instructions
• 

  32-102—Ames II™ Mutagenicity Assay Kit by BioReliance™

  Includes materials required to perform a microtiter plate fluctuation test using Salmonella typhimurium TA98 and TAMix (TAMix consists of a mixture of strains TA7000 through TA7006). Mutations arising due to frame shift events are detected by TA98. TAMix includes individual TA7000 series strains responsive to each of the several possible base-pair substitution events. More tools here >>
  
  
  • STDisc™ TA98 and TAMix (32-71098 and 32-71001, 2 discs/strain/vial)
  • Ampicillin (32-26007, 50mg)
  • Growth Medium (32-26003, 100ml)
  • Exposure Medium (32-26001, 100ml)
  • Indicator Medium (32-26005, 300ml)
  • MUTAZYME™ - 30% S9 Mix (11-401.3L, 3.25ml)
  • 4-Nitroquinoline-N-Oxide (32-60127, 50µg)
  • 2-Nitrofluorene (32-60111, 100µg)
  • 2-Aminoanthracene (32-60107, 125µg)
  • Software disc (32-90002) and instruction manual.

• UMU Genotoxicity Test Kit

  31-400 – UMU Genotoxicity Test Kit. Consists of materials to perform the umu genotoxicity assay as described by Reifferscheid, G, et al., “A microplate version of the SOS/umu-test for rapid detection of genotoxins and genotoxic potentials of environmental samples”, Mutation Research, 253:215-222, 1991 and codified in ISO 13829. More tools here >>
  
  
  • TGA-Culture Medium (26-714.1 x 2)
  • 10X TGA-Culture Medium (26-715, 10ml)
  • Ampicillin (22-147, 50mg)
  • B-buffer (26-716, 35ml )
  • Stop Reagent (26-718, 30ml)
  • ONPG (22-148L, 4.95mg x 2)
  • 2-Mercaptoethanol (22-149, 100 µl x 2)
  • 4-Nitroquinoline-N-oxide (60-163, 12.5µg)
  • 2-Aminoanthracene (60-164, 50µg x 2)
  • MUTAZYME™ 30% S9 Mix (11-401.3L, 3.25ml)
  • PTM^™ Salmonella typhimurium TA1535pSK1002 (73-1535pSK, 2 vials)
  • User Instructions

• Moltox FT™ Culture Media

  • Moltox FT™ Growth Medium

    26-712.05	50ml
    26-712.1	100ml
    26-712.5	500ml

  • Moltox FT™ Exposure Medium

    26-710.05	50ml
    26-710.1	100ml
    26-710.5	500ml

  • Moltox FT™ Reversion Indicator Medium

    26-711.1	100ml
    26-711.3	300ml
    26-711.5	500ml

• Ames II™ by BioReliance Culture Media

  
  • Growth Medium

    32-26003	100ml
    32-26004	1000ml

  • Exposure Medium

    32-26001	100ml
    32-26002	1000ml

  • Indicator Medium

    32-26005	300ml
    32-26006	1000ml

• UMU Genotoxicity Test Culture Media and Reagents

  • TGA – Culture Medium

    26-714	100ml
    26-714.25	250ml
    26-714.5	500ml

  • 10X TGA-Culture Medium

    26-715	10ml
    26-715.05	50ml

  • B-Buffer

    26-716	35ml
    26-716.1	100ml

  • Stop Reagent

    26-718	30ml
    26-718.1	100ml

  • ONPG (Lyophilized in 0.1m phosphate buffer, pH 7.4)

    22-148

    1.1ml

  • 2-Mercaptoethanol

    22-149

    100µl