S9

• Lyophilized S9 Products

  Freeze dried using a proprietary process that confers exceptional stability. Especially useful where an ultralow freezer is not available and where dry ice shipments are difficult or prohibitively expensive. Ready for use after reconstitution with the label volume of cold, sterile, purified water.

  • 11-01L.2 – Aroclor 1254 Induced male Sprague Dawley rat liver S9, in 0.15M KCl, 2 ml
  • 11-05L.2 – Phenobarbital/5, 6 Benzoflavone Induced male Sprague Dawley rat liver S9, in 0.15M KCl, 2 ml
  • 15-03SL.5 – Aroclor 1254 Induced male Golden Syrian hamster liver S9, in 0.15M KCl, 5 ml

  Store in standard freezer

• Frozen S9 Preparations

  • 11-101.1	1 ml
    11-101.2	2 ml
    11-101.5	5 ml

    11-101 – Aroclor 1254 Induced male Sprague Dawley rat liver S9, in 0.15M KCl. This is the most commonly used S9. Prepared using 50mg/kg Aroclor 1254-inductions after Maron & Ames (Mutat res, 113:173, 1983).

  • 11-102.2	2 ml
    11-102.5	5 ml

    11-102 – Uninduced male Sprague Dawley rat liver S9, in 0.15M KCl

  • 11-105.2	2 ml
    11-105.5	5 ml

    11-105 – Phenobarbital/5,6 Benzoflavone Induced male Sprague Dawley rat liver S9, in 0.15m KCl. Prepared using the treatment schedule of Matsushima, et. al., In: In vitro metabolic activation in mutagenesis testing (F.J. de Serres, et al., eds.) Elsevier/North Holland, 1976, p. 58.

  • 11-111.2	2 ml
    11-111.5	5 ml

    11-111—Aroclor 1254-induced male Sprague Dawley rat liver S9. In 0.25M sucrose. Special order.
  • 11-115—Ethanol-induced male Sprague Dawley rat liver S9. Special order.
  • 11-116—Phenobarbital-induced male Sprague Dawley rat liver S9. Special order.
  • 11-117—5,6-benzoflavone-induced male Sprague Dawley rat liver S9. Special order.
  • 12-107—Aroclor 1254-induced male Fisher 344 rat liver S9. Special order.
  • 12-109—Uninduced male Fisher 344 rat liver S9. Special order.

  • 14-103.5	5 ml
    14-103.1	1 ml

    14-103—Pooled Human liver S9, in 0.15M KCl.

  • 15-03S.5

    5 ml

    15-03S—Aroclor 1254 Induced male Golden Syrian hamster liver S9, in 0.15m KCl. Specifically prepared for use in the “Modified” Ames test for petroleum oils. Quality Control data includes triplicate plate assay against the standard reference oil.
  • 15-104—Uninduced male Golden Syrian Hamster liver S9. In 0.15M KCl. For use in control experiments or for application in the “reductive” protocol (Prival & Mitchell, Mutat res, 97:103, 1982).
  • 16-201—B6C3F1 male mouse liver S9. Aroclor 1254-induced. In 0.15M KCl. Special order.
  • 16-202—B6C3F1 male mouse liver S9. Uninduced. In 0.15M KCl. Special order.
  • 16-401—CD-1 male mouse liver S9. Aroclor 1254-induced. Special order.
  • 16-402—CD-1 male mouse liver S9. Uninduced. Special order.

Custom Preparations

Our laboratory has extensive experience preparing S9’s and microsomes from various species, tissues and after inductions with a wide range of agents. We would be glad to have the opportunity to work with you to meet your particular requirements. Minimum order may apply.

Microsomes can be prepared according to your specifications.

• • 18-101 - Aroclor 1254 Induced male Sprague Dawley rat liver Microsomes, in Sucrose/Tris/EDTA buffer. Prepared by isoelectric precipitation.

Regensys ™ NADPH Regenerating System Reagents

• Regenerating Systems

  • 60-200.15	15 ml
    60-200.4	40 ml
    60-200.5	50 ml

    60-200 - NADPH Regensys™ A
    Contains Glucose-6-Phosphate, MgCl2/KCl in 0.1 M phosphate buffer, pH 7.4. For preparation of 4 – 10% S9 mix. Formulated as described by Maron & Ames (Mutat. Res. 113:173, 1983). Complete the system with the addition of corresponding Regensys^™ B & S9. Fill sizes refer to final volume of S9 mix per package. Store in refrigerator.)

  • 60-201.15L	46 mg
    60-201.4L	123 mg
    60-201.5L	153 mg

    60-201L – NADPH Regensys^™ B
    Consists of lyophilized NADPH to complete the Regensys^™ system--add RegensysTM B & appropriate volume of S9 to the corresponding Regensys^™ A. Store in freezer.)

• MUTAZYME™

  Aroclor 1254-induced Sprague Dawley male rat liver S9 lyophilized with NADPH-regenerating system cofactors and phosphate buffer. To use, simply reconstitute with the label volume of ice-cold, sterile, purified water. Available as 5%, 10% and 30% S9 Mix.

  • 11-401.3L	3.25 ml
    11-401.6 L	6.5 ml

    MUTAZYME™ 30% S9 Mix Designed for use in microtiter plate-based assays such as the Moltox FT™ and UMU assays.

  • 11-402L

    20ml

    MUTAZYME™ 10% S9 Mix (Reconstitutes to 20ml.)

  • 11-403L

    20ml

    MUTAZYME™ 5% S9 Mix (Reconstitutes to 20ml.)

• Miscellaneous Regenerating System Reagents –

  • 26-510.039	100 ml
    26-510.047	500 ml

    26-510 – Sodium Phosphate Buffer; 0.1 M, pH 7.4
  • 26-511 – Glucose-6-Phosphate; 1.0 M, 2 ml

  • 26-512.010	10 ml
    26-512.020	20 ml

    26-512 – MgCl2/KCl Solution; 0.4 M MgCl2, 1.65 M KCl