PLATED MEDIA, BROTHS AND REAGENTS FOR GENETIC TOXICOLOGY

• Minimal Glucose Agar

  • 21-400.5	Case of 500 dishes
    21-400.2	Sleeve of 20 dishes

    21-400 - Minimal Glucose Agar Dishes. Poured 30ml/dish. VBE salts. D-glucose and agar as described by Maron and Ames (Mutat res, 113:173, 1983). Packaged in heat sealed polyethylene sleeves.

  If your laboratory uses specifically modified formulations or packaging, we can provide custom media per your specification at little or no added cost. Among the various formulations available are histidine and biotin supplemented bottom and top agars as well as MGA’s manufactured using Davis rather than VBE salts or with modified D-glucose concentrations.

[Download MGA chart here]

• Regenerating System Reagents

  • 26-510.039	100 ml
    26-510.047	500 ml

    26-510 – Sodium Phosphate Buffer; 0.2 M, pH 7.4
  • 26-511 – Glucose-6-Phosphate; 1.0 M, 2 ml

  • 26-512.01	10 ml
    26-512.02	20 ml

    26-512 – MgCl2/KCl Solution; 0.4 M MgCl2, 1.65 M KCl

• Phenotype Confirmation

  • 21-199 – EC TRI PC^â„¢ – For phenotypic confirmation of E. coli WP2 strains. Tests for tryptophan auxotrophy and pKM101. 5 plates/sleeve.
  • 21-200 – ST QUAD PC^â„¢ – For phenotypic confirmation of S. typhimurium Ames strains. Tests for histidine auxotrophy, pKM101, and pAQ1. Includes crystal violet discs. 5 plates/sleeve.
  • 26-804 – Ampicillin Solution. 8 mg/ml. For use as media additive with pKM101 containing strains. 10 ml/tube.
  • 26-805 – Tetracycline Solution. 0.8 mg/ml. For use as a media additive with pAQ1 containing strains. 10 ml/tube.
  • 21-203 – Histidine/Biotin Master Plates – Minimal Glucose Agar with excess histidine and biotin. For use with non-pKM101 containing strains. 10/sleeve.
  • 21-201 – Ampicillin Master Plates – Minimal Glucose Agar with excess histidine and biotin plus ampicillin. For use with pKM101 containing strains. 5 plates/sleeve.
  • 21-202 – Tetracycline Master Plates – Minimal Glucose Agar with excess histidine, biotin, ampicillin, and tetracycline. For use with TA102 (pAQ1). 5/sleeve.
  • 26-809 – Crystal Violet Discs. For assessment of the rfa genotype found in S. typhimurium Ames strains. 10 discs/vial.

• Top Agars, Nutrient Agars & Broths and Media Additives

  • 21-100 – Nutrient Agar. Prepared with VBE and Oxoid Nutrient Broth No. 2. For general growth and CFU determinations of Ames S. typhimurium and E. coli WP2 strains. 20 plates/sleeve.

  • 26-505.05	50 ml
    26-505.1	100 ml
    26-505.3	300 ml
    26-505.5	500 ml

    26-505 – Nutrient Broth, Oxoid No. 2. For culture of S. typhimurium strains and E. coli WP2.

  • 26-555.3	300 ml
    26-555.5	100 ml

    26-555 – Nutrient Broth, Oxoid No. 2 & VBE. For culture of S. typhimurium strains and E. coli WP2.

  • 26-501.1	100 ml
    26-501.25	250 ml
    26-501.3	300 ml
    26-501.6	600 ml

    26-501 – Top Agar. 0.7% Agar. Requires supplementation with histidine/biotin. For use with Minimal Glucose Agar and S. typhimurium strains.

  • 26-502.1	100 ml
    26-502.3	300 ml

    26-502 – Tryptophan Top Agar, 0.05 mM. For use with Minimal Glucose Agar and E. coli WP2 strains.

  • 26-503.1	100 ml
    26-503.3	300 ml
    26-503.5	500 ml

    26-503— Histidine/Biotin Top Agar. 0.05mM. For use with S. typhimurium strains in the Ames assay.

  • 26-550.039	100 ml
    26-550..047	500 ml

    26-550 – VBE (Vogel-Bonner) Salts, 50X, non-sterile. For use in the manufacture of Minimal Glucose Agar plates.

  • 26-541

    140 ml

    26-541 –VBE (Vogel-Bonner) Salts, 50X, sterile. For use in the manufacture of Minimal Glucose Agar.

  • 26-551.039	100 ml
    26-551.047	500 ml

    26-551 – Glucose, 40%, Steam sterilized. For use in the manufacture of Minimal Glucose Agar.