| Salmonella Mutagenicity Assay Kit | ||
| The materials contained in this Salmonella Mutagenicity Assay Kit include virtually all of the supplies necessary for the conduct of the “Ames Assay” as described by Maron & Ames (Maron, D. M. and B. N. Ames, Revised methods for the Salmonella mutagenicity test, Mutation Research, 113: 173-215, 1983.) - we strongly recommend that you carefully read this paper before you attempt to perform the assay. | ||
| Item | Qty. / Kit | Decription |
|
11-01L.2 |
2 | Lyophilized Post-Mitochondrial Supernatant. Sprague-Dawley rat liver. Male. Aroclor 1254 induced. In 0.154M KCL. 2.1ml/vial. Store in freezer. |
| 21-100 | 1 | Nutrient Agar Plates. Oxoid #2. 30ml/plate. Room Temperature. 20/sleeve. |
| 21-200 | 1 | ST QUAD PC Plates. For phenotypic confirmation of TA strains. Tests for histidine auxotrophy pKM101 and pAQ21 presence. Refrigerate. 5/sleeve. |
| 21-400.2 | 8 | Minimal Glucose Agar Plates. 20/sleeve. Store at Room Temperature. |
| 26-503.1 | 4 | Top Agar. Supplemented with L-Histidine & D-Biotin. 100ml. Store @ Room Temperature. |
| 26-505.1 | 1 | Nutrient Broth. Oxoid #2, 100ml. Store @ Room Temperature. |
| 60-100 | 1 | ControlChem Mitomycin C. 5ug/vial. 5/pack. Refrigerate. |
| 60-101 | 1 | ControlChem ICR191 ACRIDINE. 10ug/vial. 5/pack. Store @ Room Temperature. |
| 60-102 | 1 | ControlChem Daunomycin. 60ug/vial. 5/pack. Refrigerate. |
| 60-103 | 1 | ControlChem Sodium Azide. 15ug/vial. 5/pack. Refrigerate. |
| 60-107 | 1 | ControlChem 2-Aminoanthracene. 100ug/vial. 5/pack. Refrigerate. |
| 60-200.4 | 1 | NADPH REGENSYS "A". 40ml size refers to final volume of S-9 mix per pkg. G-6-P MgC12-KCL in 0.1M phosphate buffer. pH 7.4. Refrigerate. |
| 60-201.4L | 1 | Lyophilized NADPH REGENSYS "B". 40ml size refers to final volume of S-9 mix per pkg. 123mg of crystalline NADP. Add to "A". Pkg w/dessicant. Store in Freezer. |
| 71-097L | 1 | Lyophilized STDisc TA97a. Consists of dried and stablilized S.Typhimurium strain TA97a cells. For use as culture inoculum. 10/vial. Refrigerate. |
| 71-098L | 1 | Lyophilized STDisc TA98. Consists of dried and stablilized S.Typhimurium strain TA98 cells. For use as culture inoculum. 10/vial. Refrigerate. |
| 71-100L | 1 | Lyophilized STDisc TA100. Consists of dried and stablilized S.Typhimurium strain TA100 cells. For use as culture inoculum. 10/vial. Refrigerate. |
| 71-102L | 1 | Lyophilized STDisc TA102. Consists of dried and stablilized S.Typhimurium strain TA102 cells. For use as culture inoculum. 10/vial. Refrigerate. |
| FAM002 | 1 | Ames Assay Kit Manual Please be advised that this is a guideline, not a protocol. |