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Reagents for Bacterial Mutagenesis |
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Item |
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Description |
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26-804 |
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Ampicillin
Solution. 8mg/ml in 0.02M NaOH. For use as
culture medium when growing pKM101-containing
strains. Filter sterilized - prepared as
described by Maron & Ames (Mutat. Res., 113:173,
1983).
*1 |
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26-702 |
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Biotin Solution.
0.5mM. Filter Sterilized. 100ml.
*1 |
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26-809 |
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Crystal Violet Discs. 10/vial. |
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26-542 |
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D-Glucose 20% 700ml
Filter Sterized.
*2 |
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26-565 |
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D-Glucose 20%
Sterile 800ml. Filter Sterilized.
*2 |
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26-551 |
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D-Glucose 40% .
Sterile. 100ml.
*2 |
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26-551.5 |
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D-Glucose 40% .
Sterile. 500ml.
*2 |
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26-511 |
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Glucose-6-Phosphate. 1.0M solution. Filter
sterilized. 2ml - prepared as described by Maron
& Ames (Mutat. Res., 113:173, 1983). Store at / or below -20C. |
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60-203.1 |
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Glucose-6-Phosphate. 2 gram. |
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26-700.1 |
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L-Histidine &
D-Biotin Solution. Top agar supplement. Filter
Sterilized. 100ml.
*1 |
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26-701.1 |
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L-tryptophan
solution. 0.5mM. Top agar supplement. Filter
Sterilized. 100ml.
*1 |
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26-805 |
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Tetracycline
Solution. 0.8mg/ml in 0.02M HCL. For use as
culture medium additive when growing
pAQ1containing strains. Filter sterilized -
prepared as described by Maron & Ames (Mutat.
Res., 113:173, 1983).
*1 |
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26-510.1
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Phosphate Buffer -
0.2M sodium phosphate, pH 7.4. Sterile. 100ml.
*2 |
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26-510.5
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Phosphate Buffer -
0.2M sodium phosphate, pH 7.4. Sterile. 500ml.
*2 |
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26-516.5
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Phosphate Buffer -
2.0M sodium Phosphate pH 7.4. Sterile. 500ml.
*2 |
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26-560 |
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Vogel-Bonner medium
E Salts 25X Filter Sterilized. 320ml.
*2 |
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26-541 |
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Vogel-Bonner medium
E Salts 50X. 140ml.
*2 |
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26-550 |
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Vogel-Bonner medium
E Salts 50X. Non-sterile. 100ml - prepared as
described by Maron & Ames (Mutat. Res., 113:173,
1983).
*2 |
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26-550.5 |
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Vogel-Bonner medium
E Salts 50X. Non-sterile. 500ml.
*2 |
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footnote: *1
Refrigerate. *2 Store at Room Temperature. |
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Moltox © 1986 |