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E. Coli Mutagenicity Test Kit
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The materials
contained in this E. coli Mutagenicity Assay Kit include
virtually all of the supplies necessary for the conduct of the
assay as described by Green, M.H.L., and Muriel, W.J., (Mutation
Research, 38: 3-32, 1976) - we
strongly recommend that you carefully read this paper before you
attempt to perform the assay. |
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Item |
Qty./ Kit |
Description |
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11-01L.2 |
1 |
Lyophilized
Post-Mitochondrial Supernatant. Sprague-Dawley rat liver. Male.
Aroclor 1254 induced. In 0.154M KCL. 2.1ml/vial. Store in
freezer. |
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21-100 |
2 |
Nutrient Agar
Plates. Oxoid #2. 30ml/plate. Room Temperature. 20/sleeve. |
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21-199 |
1 |
EC TRI PC
Plates. For Phenotypic confirmation of E.Coli WP2. Tests for
tryptophan auxotrophy and pKM101. Refrigerate. 5/sleeve. |
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21-400.2 |
4 |
Minimal
Glucose Agar Plates. 20/sleeve. Store at Room Temperature. |
|
26-502.1 |
1 |
Top Agar.
Supplemented with L-Tryptophan for use with the E.Coli WP2.
100ml. Store @ Room Temperature. |
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26-505.1 |
1 |
Nutrient
Broth. Oxoid #2, 100ml. Store @ Room Temperature. |
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60-107 |
1 |
ControlChem
2-Aminoanthracene. 100ug/vial. 5/pack. Refrigerate. |
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60-108 |
1 |
ControlChem
MMS - Methyl Methane-sulfonate. 25ul. 5/pack. Refrigerate. |
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60-200.15 |
1 |
NADPH
REGENSYS "A". 15ml size refers to final volume of S-9 mix per
pkg. G-6-P MgCl2-KCL in 0.1M phosphate buffer. pH 7.4.
Refrigerate. |
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60-201.15L |
1 |
Lyophilized
NADPH REGENSYS "B". 15ml size refers to final volume of S-9 mix
per pkg. 46mg of crystalline NADP. Add to "A". Pkg w/dessicant.
Store in freezer. |
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72-187L |
1 |
ECDisc WP2.
Consists of Lyophilized and stabilized E.Coli strain WP2 trp
cells. For use as culture inoculum. 10/vial. Refrigerate. |
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72-188L |
1 |
Lyophilized
ECDisc WP2 uvrA. Consists of dried and stabilized E.Coli strain
WP2 trp uvrA cells. For use as culture inoculum. 10/vial.
Refrigerate. |
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FAM004 |
1 |
E. coli Test Kit Manual Please be advised that this is a
guideline, not a protocol. |