Genotoxicity Assay Tester Strains

Most commonly employed bacterial strains for use in mutagenicity testing are provided in convenient dried disc format. A few strains are not amenable to freeze drying and are offered suspended in our proprietary transport medium. Strains were obtained from their primary source or from established repositories; for example, Salmonella strains were obtained from Dr. Bruce Ames, University of California, Berkeley; E. coli strains were obtained from the National Collections of Industrial and Marine Bacteria Limited, Aberdeen; Scotland, UK. Strains were characterized for their diagnostic phenotypes upon receipt. Confirmed strains were lyophilized, dried and stabilized using a modification of the method of Obara, et. al., J. Clinical Microbiology, 14:61-66, 1981 or suspended and frozen in transport medium. After preservation, samples were cultured and their phenotypes and responses to diagnostic mutagens again determined. Strains are accompanied by application and Quality Control statements that include the results of phenotypic confirmation and diagnostic mutagen response studies.

Please Note: The bacterial strains contained in STDiscsâ„¢ and ECDiscsâ„¢ are potential etiologic agents and are intended for use only by those skilled in the safe handling of potentially infectious agents.

• STDiscsâ„¢

  Salmonella typhimurium strains for use in the bacterial mutagenesis assay described by Maron, D. and Ames., B., Mutation Research, 113:173-215, 1983.

  • 71-097aL	STDiscâ„¢ TA97a	(hisD6610, hisO1242, uvrB, rfa, pKM101)	10 discs
    71-098L	STDiscâ„¢ TA98	(hisD3052, uvrB, rfa, pKM101)	10 discs
    71-100L	STDiscâ„¢ TA100	(hisG46, uvrB, rfa, pKM101)	10 discs
    71-102L	STDiscâ„¢ TA102	(hisG428, rfa, pKM101, pAQ1)	10 discs
    71-1535	STDiscâ„¢ TA1535	(hisG46, uvrB, rfa	10 discs
    71-1537	STDiscâ„¢ TA1537	(his3076, uvrB, rfa)	10 discs
    71-1538	STDiscâ„¢ TA1538	(hisD3052, uvrB, rfa)	10 discs

• ECDiscsâ„¢

  Escherichia coli strains (WP2 derivatives) for use in the bacterial mutagenesis assay described by Green, M.H.L. and Muriel, W.J., Mutation Research, 38:3-32, 1976.

  • 72-187L	ECDiscâ„¢ WP2	(trp)	10 discs
    72-188L	ECDiscâ„¢ WP2	(trp, uvrA)	10 discs
    72-002L	ECDiscâ„¢ WP2	(trp, pKM101)	10 discs
    72-003L	ECDiscâ„¢ WP2	(trp, urvA pKM101)	10 discs

• UMU

  Salmonella strain for use in the SOS/umu-test described by Oda, Y., S. Nakamura, J. Oki and T. Kato, Mutation Research, 147:219-119, 1985. Offered in proprietary transport medium (PTMâ„¢).

  • 73-1535pSK

    PTMâ„¢ TA1535pSK

    (hisG46, uvrB, rfa, pSK1002)

    per PTMâ„¢