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Bacterial Tester Strains   Bacterial Tester Strains

Genotoxicity Assay Tester Strains

Most commonly employed bacterial strains for use in mutagenicity testing are provided in convenient lyophilized disc format. A few strains are not amenable to freeze drying and are offered either suspended transport medium or frozen. Strains were obtained from their primary source or from established repositories; for example, Salmonella strains were obtained from Dr. Bruce Ames, University of California, Berkeley; E. coli strains were obtained from the National Collections of Industrial and Marine Bacteria Limited, Aberdeen; Scotland, UK. Strains were characterized for their diagnostic phenotypes upon receipt. Confirmed strains were lyophilized, in modified ATCC Reagent 18, or suspended in transport medium (73-1535psk), or frozen (TAMix). After preservation, samples were cultured and their phenotypes and responses to diagnostic mutagens again determined. Strains are accompanied by Quality Control statements that include the results of phenotypic confirmation, diagnostic mutagen response and viability studies. Culture instructions are included with the QC Statement. Note1: The bacterial strains contained in STDiscs™ and ECDiscs™ are potential etiologic agents and are intended for use only by those skilled in the safe handling of potentially infectious agents.

STDiscs™

Salmonella typhimurium strains for use in the bacterial mutagenesis assay described by Maron, D. and Ames., B., Mutation Research, 113:173-215, 1983.
Part # Description Size Master Pack  
71-097L 71-097aL -- STDisc™ TA97a (hisD6610, hisO1242, uvrB, rfa, pKM101), 10 discs. 10 discs S9 and Metabolic Activation
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71-098L 71-098L -- STDisc™ TA98 (hisD3052, uvrB, rfa, pKM101), 10 discs. 10 discs S9 and Metabolic Activation
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71-100L 71-100L -- STDisc™ TA100 (hisG46, uvrB, rfa, pKM101), 10 discs. 10 discs S9 and Metabolic Activation
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71-102L 71-102L -- STDisc™ TA102 (hisG428, rfa, pKM101, pAQ1), 10 discs. 10 discs S9 and Metabolic Activation
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71-1535 71-1535 -- STDisc™ TA1535 (hisG46, uvrB, rfa), 10 discs. 10 discs S9 and Metabolic Activation
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71-1537 71-1537 -- STDisc™ TA1537 (his3076, uvrB, rfa), 10 discs. 10 discs S9 and Metabolic Activation
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71-1538 71-1538 -- STDisc™ TA1538 (hisD3052, uvrB, rfa), 10 discs. 10 discs S9 and Metabolic Activation
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ECDiscs™

Escherichia coli strains (WP2 derivatives) for use in the bacterial mutagenesis assay described by Green, M.H.L. and Muriel, W.J., Mutation Research, 38:3-32, 1976.
Part # Description Size Master Pack  
72-002L 72-002L -- ECDisc™ WP2 (trp, pKM101), 10 discs. 10 discs S9 and Metabolic Activation
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72-003L 72-003L -- ECDisc™ WP2 (trp, urvA, pKM101), 10 discs. 10 discs S9 and Metabolic Activation
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72-187L 72-187L -- ECDisc™ WP2 (trp), 10 discs. 10 discs S9 and Metabolic Activation
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72-188L 72-188L -- ECDisc™ WP2 (trp, uvrA), 10 discs. 10 discs S9 and Metabolic Activation
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